ABSTRACT
Introduction and Aims: matrix metallo proteinase-2 [MMP-2] is a family of proteolytic enzymes involved in degrading and remodeling the extracellular matrix and basement membrane. Researchers have shown that Mast cells have an important role in the inflammatory disease including lung fibrosis and they can be as the source of MMP-2 protein. The aim of this research is the examination of Thalidomide effect on the MMP-2 protein expression and Mast cells in the lung fibrosis induced by Bleomycin in mice
Materials and Methods: in this research, 32 male adult C57BL/6 mices were randomly divided into 4 groups. Mices received in group 1 [Bleomycin] Bleomycin sulfate, in group 2 [Bleomycin+Thalidomide] Bleomycin beside Thalidomide, in group 3[Thalidomide] Thalidomide and in group 4 [Carboxy Methyl Cellulose] Carboxy Methyl Cellulose via intraperitoneum. At the end of experiment, mice's lung samples were prepared and histological and immunohistochemical studies were performed about them. After the investigation of tissue slides, number of MMP-2 protein expression cells and Mast cells were calculated and results were analyzed by using OneWay ANOVA and Tukey's test
Results: histological and immunohistochemical studies showed a significant increase in the number of MMP-2 protein expression and Mast cells in the Bleomycin group in comparison with the Carboxy Methyl Cellulose group [p < 0.001]. But number of these cells in the Bleomycin+Thalidomide group decreased significantly compared to the Bleomycin group [p < 0.001]
Conclusion: the results showed that the number of expressive cells of MMP-2 protein and Mast cells decreases by Thalidomide and subsequently reduces lung fibrosis in the mice
ABSTRACT
Misdiagnosing ovarian torsion is now suggested as an important issue in clinical setting. The aim of this study was to determine the diagnostic accuracy of sonography for ovarian torsion. In this study 323 women with acute pelvic pain with highly suspected ovarian torsion signs and symptoms attending Imam Reza Medical Center in Kermanshah between 2011 through 2012 were included and underwent a transabdominal sonography [2-5 MHz probes]. Then findings of sonography were compared with laparatomy. The ultrasound correctly diagnosed 72.1% of ovarian torsion and missed 27.9% of them [false negatives]. However, one free subject [0.4%] was misclassified as ovarian torsion [false positive]. There was a strong correlation between sonography and laparatomy with a kappa value of 84.0%. The sensitivity and specificity of sonography were 72.1% and 99.6%, respectively. Sonography had a positive predictive value of 96.9%, a negative predictive value of 95.9%, and a total accuracy of 96.0% for detection of ovarian torsion. Sonography appears to be an excellent method to evaluate patients with suspected ovarian torsion. Abnormal blood flow detected by sonography is highly predictive of ovarian torsion and is therefore useful in the diagnosis of this phenomenon
ABSTRACT
Evaluating the expression of Oct-4, NANOG, Sox2 and Nucleostemin in colon cancer cell lines [Caco-2 and HT-29]. Caco-2 and HT-29 human colon cancer cell lines were cultured in Dulbecco's modified eagles medium [DMEM] and Roswell Park Memorial Institute medium [RPMI] respectively, containing 10% fetal bovin serum [FBS] with 1% peniciline and streptomycinen in 37°, 5% CO2 incubator. Total RNA was isolated using the ISOGEN method. RNA integrity was checked with the use of agarose gel electrophoresis and spectrophotometry. Reverse transcriptase polymerase chain reaction [RT-PCR] was used to examin the samples. The expression of Oct-4 and Nucleostemin at the protein level was further determined using immunocytochemistry. RT-PCR analysis of Caco2 and HT-29 colon cancer cell lines showed expression of Oct-4, NANOG, Sox2 and Nucleostemin genes. Also immunocytochemical analysis confirmed the cytoplasmic and nuclear expression of the Oct-4 protein and Nucleostemin proteins. Collectively, our data confirmed the expression of Oct-4, NANOG, Sox2 and Nucleostemin in colon cancer cells and suggested that their expression can be used as potential tumor markers in diagnosis and /or prognosis of colon tumors. These results confirm the potential value of the cancer stem-cell theory in cancer therapy